Three-Dimension Resolution Enhanced Microscopy Based on Parallel Detection
نویسندگان
چکیده
منابع مشابه
Three-dimensional resolution and contrast-enhanced confocal microscopy with array detection.
What we believe is a novel method for improving confocal microscopy's resolution and contrast in 3D space is proposed. Based on a conventional confocal microscopy setup, we use an array detector composed of 32 photomultiplier tubes (PMTs) to replace one point-detector, where the location offset of each PMT caused a different effective point spread function (PSF). By applying array detection and...
متن کاملMirror-enhanced super-resolution microscopy
Axial excitation confinement beyond the diffraction limit is crucial to the development of next-generation, super-resolution microscopy. STimulated Emission Depletion (STED) nanoscopy offers lateral super-resolution using a donut-beam depletion, but its axial resolution is still over 500 nm. Total internal reflection fluorescence microscopy is widely used for single-molecule localization, but i...
متن کاملExpression-Enhanced Fluorescent Proteins Based on Enhanced Green Fluorescent Protein for Super-resolution Microscopy.
"Smart fluorophores", such as reversibly switchable fluorescent proteins, are crucial for advanced fluorescence imaging. However, only a limited number of such labels is available, and many display reduced biological performance compared to more classical variants. We present the development of robustly photoswitchable variants of enhanced green fluorescent protein (EGFP), named rsGreens, that ...
متن کاملMobile Device-based Three-Dimension Coordinate Estimation Using Face Detection
In this paper, we propose a 3D coordinate estimation for 3D space using a mono camera in mobile devices. Generally, a marker is used for the detection of 3D movement in 2D images. However, this marker-based system has obvious defects since markers always need to be included in the image. To overcome this problem, we replaced the markers with a planar face by estimating the face detection. The e...
متن کاملAtomic resolution three-dimensional electron diffraction microscopy.
We report the development of a novel form of diffraction-based 3D microscopy to overcome resolution barriers inherent in high-resolution electron microscopy and tomography. By combining coherent electron diffraction with the oversampling phasing method, we show that the 3D structure of a nanocrystal can be determined ab initio at a resolution of 1 A from 29 simulated noisy diffraction patterns....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied Sciences
سال: 2021
ISSN: 2076-3417
DOI: 10.3390/app11062837